Fluorescence microscopy showing co-localisation of (A) CD59 and (B) β1 integrin with purified MBP-fusion proteins on HEp-2 cells. Cells were incubated with MBP-Ifp or MBP-IfpC337G. MBP-fusion proteins were visualised with anti-Ifp and anti-rabbit Alexafluor 594 antibodies. CD59 was visualised with anti-CD59 and anti-mouse Alexafluor 488 antibodies. β1 integrin was visualised with anti-β1 integrin and anti-mouse Alexafluor 488 antibodies. Representative cells are shown.