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Table 2 Effect of macrophage-culture grown E. chaffeensis whole-cell lysate on the transcription of p28-Omp14 (pRG147) and p28-Omp19 (pRG198) promoter constructs quantitated by densitometry and real-time RT-PCR (fold change)

From: Isolation and characterization of Ehrlichia chaffeensis RNA polymerase and its use in evaluating p28 outer membrane protein gene promoters

  Densitometry# Real-time RT-PCR
Template no lysate
(x ± std)
with lysate
(x ± std)
fold increase p-value* no lysate
(Ct ± std)
with lysate
(Ct ± std)
fold increase p-value*
pRG147 83.3 ± 1.8 45.5 ± 1.9 1.8 <0.0001 17.51 ± 0.81 16.64 ± 0.23 1.81 0.0174
pRG198 76.9 ± 1.7 35.7 ± 1.6 2.2 <0.0001 17.48 ± 0.08 16.27 ± 0.06 2.24 0.0013
  1. #volume of the unoccupied space available under the signal is quantitated
  2. *p-value of ≤ 0.05 is significant