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Table 2 Effect of macrophage-culture grown E. chaffeensis whole-cell lysate on the transcription of p28-Omp14 (pRG147) and p28-Omp19 (pRG198) promoter constructs quantitated by densitometry and real-time RT-PCR (fold change)

From: Isolation and characterization of Ehrlichia chaffeensis RNA polymerase and its use in evaluating p28 outer membrane protein gene promoters

 

Densitometry#

Real-time RT-PCR

Template

no lysate

(x ± std)

with lysate

(x ± std)

fold increase

p-value*

no lysate

(Ct ± std)

with lysate

(Ct ± std)

fold increase

p-value*

pRG147

83.3 ± 1.8

45.5 ± 1.9

1.8

<0.0001

17.51 ± 0.81

16.64 ± 0.23

1.81

0.0174

pRG198

76.9 ± 1.7

35.7 ± 1.6

2.2

<0.0001

17.48 ± 0.08

16.27 ± 0.06

2.24

0.0013

  1. #volume of the unoccupied space available under the signal is quantitated
  2. *p-value of ≤ 0.05 is significant
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BMC Microbiology

ISSN: 1471-2180

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