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Table 3 Herbaspirillum seropedicae strains and plasmids

From: Role of PII proteins in nitrogen fixation control of Herbaspirillum seropedicae strain SmR1

Strains Phenotype/genotype Reference
Herbaspirillum seropedicae  
SmR1 Wild type, Nif+, SmR [38]
LNglnK SmR1 containing glnK::sacB- KmR this work
LNglnKdel SmR1 containing ΔglnK this work
LNglnB SmR1 containing glnB::TcR this work
LNamtBlacZ SmR1 containing amtB::lacZ-KmR this work
LNglnKamtBlacZ LNglnKdel containing amtB::lacZ-KmR this work
LNglnBamtBlacZ LNglnB containing amtB::lacZ-KmR this work
B12-27 SmR1 containing glnB::Tn5-20B [14]
Escherichia coli   
DH10B Smr; F' [proAB+ lacZΔM15] Life Technologies
S17.1 SmR, Tra+ pro thi recA hsdR (RP4-2 kan::Tn7 tet::Mu) [39]
Plasmids Relevant characteristics Reference
pACB192 1.7 kb DNA fragment containing the glnB gene of H. seropedicae in pSUP202 This work
pACB194 glnB gene of H. seropedicae with a tetracycline resistance transposon EZ::TN™ < TET-1 > (Epicentre) in pSUP202 this work
pACB210 glnB gene of H. seropedicae in pLAFR3.18Cm this work
pDK6 Expression vector/tac promoter, KmR [37]
pDK6nifACT H. seropedicae nifA deleted of 606 bp in the 5'coding region cloned into pDK6 carrying the nifA promoter this work
pDK6pnifA nifA gene promoter region of H. seropedicae in pDK6 this work
pEMS140 nifB-lacZ transcriptional fusion of H. seropedicae in pPW452 [21]
pEMS301 1.7 kb EcoRI fragment that contains the promoter region and part of the nifA gene of H. seropedicae in pTZ19R [40]
pLAFR3.18Cm TcR, CmR, IncP cosmid with the pTZ18R cloning nest [15]
pLNΔNifA Expresses ΔN-NifA of H. seropedicae with its own promoter in pLAFR3.18Cm this work
pLNOGA 5.1 kb fragment that contains the nlmAglnKamtB operon of H. seropedicae in pLAFR3.18Cm (former named pLARF3.18OGA) [15]
pLNglnK 0.9 kb BamHI/HindIII fragment that contains the 3' terminal of the nlmA gene, the complete glnK gene and 5' terminal of the amtB gene of H. seropedicae in pTZ18R this work
pMH1701 KmR, contains a sacB-KmR cassette [35]
pPW452 TcR, transcriptional lacZ gene fusion [41]
pRAM2T7 contains H. seropedicae nifA deleted of 606 bp in the 5'end, encoding an N-truncated form of NifA deleted of its N-terminal domain and Q-linker this work
pRAMM1 nifA of H. seropedicae in pLAFR3.18Cm this work
pRW1 nifA-lacZ transcriptional fusion of H. seropedicae in pPW452 [20]
pSUP202 ApR, CmR, TcR, Mob [39]
pSUPamtBClacZ Central region of the amtB gene with a lacZ-KmR cassette insertion in pSUP202 [15]
pSUPglnK 0.9 kb BamHI/HindIII fragment that contains the 3' terminal of the nlmA gene, the complete glnK gene and 5' terminal of the amtB gene of H. seropedicae in pSUP202 this work
pSUPglnKdel ΔglnK (192bp) gene of H. seropedicae in pSUP202 this work
pSUPglnKdelsacB contains ΔglnK and a sacB-KmR cassette (from pMH1701) cloned into the vector pSUP202 this work
pSUPglnKsacB 0.9 kb fragment spanning from the 3'end of nlmA to the 5'end of amtB with a sacB-KmR (from pMH1701) inserted into the glnK gene this work
pTZ19R ApR lacZ f1 IG [42]
pUC18 ApR, lacZ, f1 Invitrogen
pUCG08del 0.8 kb DNA fragment that contains the 3' terminal of the nlmA gene, the complete glnK gene and the 5' terminal of the amtB gene of H. seropedicae in pUC18. this work
pUCglnKdel ΔglnK gene of H. seropedicae in pUC18 this work