Table 3 Herbaspirillum seropedicae strains and plasmids

Herbaspirillum seropedicae

SmR1

Wild type, Nif⁺, Sm^R

[38]

LNglnK

SmR1 containing glnK::sacB- Km^R

this work

LNglnKdel

SmR1 containing ΔglnK

this work

LNglnB

SmR1 containing glnB::Tc^R

this work

LNamtBlacZ

SmR1 containing amtB::lacZ-Km^R

this work

LNglnKamtBlacZ

LNglnKdel containing amtB::lacZ-Km^R

this work

LNglnBamtBlacZ

LNglnB containing amtB::lacZ-Km^R

this work

B12-27

SmR1 containing glnB::Tn5-20B

[14]

Escherichia coli

DH10B

Sm^r; F^' [proAB⁺ lacZΔM15]

Life Technologies

S17.1

Sm^R, Tra⁺ pro thi recA hsdR (RP4-2 kan::Tn7 tet::Mu)

[39]

pACB192

1.7 kb DNA fragment containing the glnB gene of H. seropedicae in pSUP202

This work

pACB194

glnB gene of H. seropedicae with a tetracycline resistance transposon EZ::TN™ < TET-1 > (Epicentre) in pSUP202

this work

pACB210

glnB gene of H. seropedicae in pLAFR3.18Cm

this work

pDK6

Expression vector/tac promoter, Km^R

[37]

pDK6nifACT

H. seropedicae nifA deleted of 606 bp in the 5'coding region cloned into pDK6 carrying the nifA promoter

this work

pDK6pnifA

nifA gene promoter region of H. seropedicae in pDK6

this work

pEMS140

nifB-lacZ transcriptional fusion of H. seropedicae in pPW452

[21]

pEMS301

1.7 kb EcoRI fragment that contains the promoter region and part of the nifA gene of H. seropedicae in pTZ19R

[40]

pLAFR3.18Cm

Tc^R, Cm^R, IncP cosmid with the pTZ18R cloning nest

[15]

pLNΔNifA

Expresses ΔN-NifA of H. seropedicae with its own promoter in pLAFR3.18Cm

this work

pLNOGA

5.1 kb fragment that contains the nlmAglnKamtB operon of H. seropedicae in pLAFR3.18Cm (former named pLARF3.18OGA)

[15]

pLNglnK

0.9 kb BamHI/HindIII fragment that contains the 3' terminal of the nlmA gene, the complete glnK gene and 5' terminal of the amtB gene of H. seropedicae in pTZ18R

this work

pMH1701

Km^R, contains a sacB-Km^R cassette

[35]

pPW452

Tc^R, transcriptional lacZ gene fusion

[41]

pRAM2T7

contains H. seropedicae nifA deleted of 606 bp in the 5'end, encoding an N-truncated form of NifA deleted of its N-terminal domain and Q-linker

this work

pRAMM1

nifA of H. seropedicae in pLAFR3.18Cm

this work

pRW1

nifA-lacZ transcriptional fusion of H. seropedicae in pPW452

[20]

pSUP202

Ap^R, Cm^R, Tc^R, Mob

[39]

pSUPamtBClacZ

Central region of the amtB gene with a lacZ-Km^R cassette insertion in pSUP202

[15]

pSUPglnK

0.9 kb BamHI/HindIII fragment that contains the 3' terminal of the nlmA gene, the complete glnK gene and 5' terminal of the amtB gene of H. seropedicae in pSUP202

this work

pSUPglnKdel

ΔglnK (192bp) gene of H. seropedicae in pSUP202

this work

pSUPglnKdelsacB

contains ΔglnK and a sacB-Km^R cassette (from pMH1701) cloned into the vector pSUP202

this work

pSUPglnKsacB

0.9 kb fragment spanning from the 3'end of nlmA to the 5'end of amtB with a sacB-Km^R (from pMH1701) inserted into the glnK gene

this work

pTZ19R

Ap^R lacZ f1 IG

[42]

pUC18

Ap^R, lacZ, f1

Invitrogen

pUCG08del

0.8 kb DNA fragment that contains the 3' terminal of the nlmA gene, the complete glnK gene and the 5' terminal of the amtB gene of H. seropedicae in pUC18.

this work

pUCglnKdel

ΔglnK gene of H. seropedicae in pUC18

this work