Figure 3
Quantitative real time-PCR analysis of select genes. Mean log2 expression for (A) representative dosR regulon genes, (B) cell cycle discriminant genes and (C) sigma factors in the ssd merodiploid M. tuberculosis strain compared to M. tuberculosis control strain. Data are mean values ± SD from independent biological samples. Ratios were calculated using the total number of gene targets from the ssd merodiploid M. tuberculosis strain or ssd::Tn mutant M. tuberculosis strain compared to paired M. tuberculosis control stain.