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Table 2 Primers used in assay of isolates for resistance determinants

From: Distribution and characterization of ampicillin- and tetracycline-resistant Escherichia colifrom feedlot cattle fed subtherapeutic antimicrobials

Gene PCR primer sequence 5'-3' a Amplicon size (bp) Genbank accession no. Control plasmid/gDNA Source of plasmid and reference
tet(A) GCT ACA TCC TGC TTG CCT TC 210 X61367 pSL18 [25]
  CAT AGA TCG CCG TGA AGA GG     
tet(B) TTG GTT AGG GGC AAG TTT TG 659 J01830 pRT11 [25]
  GTA ATG GGC CAA TAA CAC CG     
tet(C) CTT GAG AGC CTT CAA CCC AG 418 J01749 pBR322 [25]
  ATG GTC GTC ATC TAC CTG CC     
tet(D) AAA CCA TTA CGG CAT TCT GC 787 L06798 pSL106 [25]
  GAC CGG ATA CAC CAT CCA TC     
tet(E) AAA CCA CAT CCT CCA TAC GC 278 L06940 pSL1504 [25]
  AAA TAG GCC ACA ACC GTC AG     
tet(G) GCT CGG TGG TAT CTC TGC TC 468 S52437 pJA8122 [25]
  AGC AAC AGA ATC GGG AAC AC     
tet(K) TCG ATA GGA ACA GCA GTA 169 S67449 PAT102 [25]
  CAG CAG ATC CTA CTC CTT     
tet(L) TCG TTA GCG TGC TGT CAT TC 267 U17153 pVB.A15 [55]
  GTA TCC CAC CAA TGT AGC CG     
tet(M) GTG GAC AAA GGT ACA ACG AG 406 X90939 pJ13 [25]
  CGG TAA AGT TCG TCA CAC AC     
tet(O) AAC TTA GGC ATT CTG GCT CAC 515 Y07780 pUOA1 Taylorb
  TCC CAC TGT TCC ATA TCG TCA     
tet(S) CAT AGA CAA GCC GTT GAC C 667 C92946 pAT451 Mulvey
  ATG TTT TTG GAA CGC CAG AG     
tetA(P) CTT GGA TTG CGG AAG AAG AG 676 L20800 pJIR39 Monash Universityc
  ATA TGC CCA TTT AAC CAC GC     
tet(Q) TTA TAC TTC CTC CGG CAT CG 904 X58717 pNFD13-2 Salyersd
  ATC GGT TCG AGA ATG TCC AC     
tet(X) CAA TAA TTG GTG GTG GAC CC 468 M37699 pBS5 [56]
  TTC TTA CCT TGG ACA TCC CG     
pse-1 CGC TTC CCG TTA ACA AGT AC 419 M69058 SU01 [28]
  CTG GTT CAT TTC AGA TAG CG    gDNA  
oxa1-like AGC AGC GCC AGT GCA TCA 708 AJ009819 SU05 [26]
  ATT CGA CCC CAA GTT TCC    gDNA  
tem1-like TTG GGT GCA CGA GTG GGT 503 AF126482.1 SU07 [26]
  TAA TTG TTG CCG GGA AGC    gDNA  
  1. a Primers selected from previously published source [26, 26].
  2. b Provided by Dr.Taylor (University of Alberta, Edmonton, AB, Canada).
  3. c Provided by the Monash University (Victoria, Australia).
  4. d Provided by Dr. Salyers (University of Illinois, Urbana, USA).