Distribution and characterization of ampicillin- and tetracycline-resistant Escherichia colifrom feedlot cattle fed subtherapeutic antimicrobials

Table 2 Primers used in assay of isolates for resistance determinants

Gene	PCR primer sequence 5'-3' ^a	Amplicon size (bp)	Genbank accession no.	Control plasmid/gDNA	Source of plasmid and reference
tet(A)	GCT ACA TCC TGC TTG CCT TC	210	X61367	pSL18	[25]
	CAT AGA TCG CCG TGA AGA GG
tet(B)	TTG GTT AGG GGC AAG TTT TG	659	J01830	pRT11	[25]
	GTA ATG GGC CAA TAA CAC CG
tet(C)	CTT GAG AGC CTT CAA CCC AG	418	J01749	pBR322	[25]
	ATG GTC GTC ATC TAC CTG CC
tet(D)	AAA CCA TTA CGG CAT TCT GC	787	L06798	pSL106	[25]
	GAC CGG ATA CAC CAT CCA TC
tet(E)	AAA CCA CAT CCT CCA TAC GC	278	L06940	pSL1504	[25]
	AAA TAG GCC ACA ACC GTC AG
tet(G)	GCT CGG TGG TAT CTC TGC TC	468	S52437	pJA8122	[25]
	AGC AAC AGA ATC GGG AAC AC
tet(K)	TCG ATA GGA ACA GCA GTA	169	S67449	PAT102	[25]
	CAG CAG ATC CTA CTC CTT
tet(L)	TCG TTA GCG TGC TGT CAT TC	267	U17153	pVB.A15	[55]
	GTA TCC CAC CAA TGT AGC CG
tet(M)	GTG GAC AAA GGT ACA ACG AG	406	X90939	pJ13	[25]
	CGG TAA AGT TCG TCA CAC AC
tet(O)	AAC TTA GGC ATT CTG GCT CAC	515	Y07780	pUOA1	Taylor^b
	TCC CAC TGT TCC ATA TCG TCA
tet(S)	CAT AGA CAA GCC GTT GAC C	667	C92946	pAT451	Mulvey
	ATG TTT TTG GAA CGC CAG AG
tetA(P)	CTT GGA TTG CGG AAG AAG AG	676	L20800	pJIR39	Monash University^c
	ATA TGC CCA TTT AAC CAC GC
tet(Q)	TTA TAC TTC CTC CGG CAT CG	904	X58717	pNFD13-2	Salyers^d
	ATC GGT TCG AGA ATG TCC AC
tet(X)	CAA TAA TTG GTG GTG GAC CC	468	M37699	pBS5	[56]
	TTC TTA CCT TGG ACA TCC CG
pse-1	CGC TTC CCG TTA ACA AGT AC	419	M69058	SU01	[28]
	CTG GTT CAT TTC AGA TAG CG			gDNA
oxa1-like	AGC AGC GCC AGT GCA TCA	708	AJ009819	SU05	[26]
	ATT CGA CCC CAA GTT TCC			gDNA
tem1-like	TTG GGT GCA CGA GTG GGT	503	AF126482.1	SU07	[26]
	TAA TTG TTG CCG GGA AGC			gDNA

ISSN: 1471-2180