Table 2 Genomic region, primers, and melting temperatures for all genes investigated

Housekeeping Genes

16S rRNA

16S ribosomal subunit

16S-For

CTGAGAATTTGATCTTGG

52°C

1549 bp

16S-Rev

AAAGGAGGTGATCCAGC

16S/23S

16S-23S intergenic spacer

Spacer-For

AAGGATAAGGAAAGCTATCA

54°C

225 bp

intergenic spacer

Spacer-Rev

AATTTTTGATCCATGCAAGA

Membrane Proteins

ompA

Outer membrane protein A

1

ompA-For

ATGAAAAAACTCTTAAAATCGG

56°C

1170 bp

ompA-Rev

TTAGAATCTGCATTGAGCAG

2

MJFvd3^a

GGITG(CT)GCAACTTTAGGIGC

50°C

457 bp

MJRvd4^a

CACAAGCTTTTCTGGACTTC

3

CpeNTVD3^b

GTTCTTTCTAACGTAGC

46°C

359 bp

CpeNTVD4^b

TGAAGAGAAACAATTTG

omcB

Cysteine-rich outer

omcB-For

ATGACCAAACTCATCAGAC

54°C

1675 bp

membrane protein B

omcB-Rev

TTAATACACGTGGGTGTTTT

pmpD

Polymorphic membrane

pmpD-For

ATGATCAGTCATATACGGAC

56°C

4145 bp

protein D

pmpD-Rev

TTAGAAAATCACGCGTACG

incA

Inclusion membrane

incA-S-F^c

TATCGTAATACCAAACCACT

52°C

984 bp

protein A

incA-S-R^c

GTGTGAGATGGCTCTTTATG

copN

Chlamydia outer protein N

copN-For

ATGGCAGCTGGAGGGAC

56°C

1191 bp

copN-Rev

TTATGACCAGGGATAAGGTT

Potential Virulence Genes

tarP

Translocated actin-recruiting phosphoprotein

1

tarP-For

ATGACCTCTCCTATTAATGG

56°C

2604 bp

tarP-Rev

CTAGTTAAAATTATCTAAGGTTT

2

tarP-2-For

AAGAACCAACTCTGCATTATGAAGAGG

54°C

768 bp

tarP-2-Rev

AAGAGGTATTCACGCGACTTCCG

MACPF

Membrane-attack

MAC-For

TTGGCGATTCCTTTTGAAGC

58°C

2346 bp

complex/perforin protein

MAC-Rev

TTATAAGCACACACTAGGTCT

ORF663

Hypothetical protein

663-F^c

AAACAACTGCACCGCTCTCT

55°C

1167 bp

663-R^c

GAAGGACTTTCTGGGGGAAG