Detection and function of an intramolecular disulfide bond in the pH-responsive CadC of Escherichia coli

BMC Microbiology

Table 2 Plasmids used in this study.

Plasmid	Relevant genotype	Source or reference
pET16b	Expression vector, Ap^r	Novagen
pET16b-cadC	cadC in pET16b	[6]
pET16b-cadC_C172A	Amino acid exchange C172A in cadC,	This work
	cadC_C172A in pET16b
pET16b-cadC_C208A	cadC_C208A in pET16b	This work
pET16b-cadC_C272A	cadC_C272A in pET16b	This work
pET16b-cadC_C172A,C208A	cadC_C172A,C208A in pET16b	This work
pET16b-cadC_C172A,C272A	cadC_C172A,C272A in pET16b	This work
pET16b-cadC_C208A,C272A	cadC_C208A,C272A in pET16b	This work
pET16b-cadC_C172A,C208A,C272A	cadC_C172A,C208A,C272A in pET16b	This work
pET16b-cadC_C208D,C272K	cadC_C208D,C272K in pET16b	This work
pET16b-cadC_C208K,C272D	cadC_C208K,C272D in pET16b	This work
pET16b-cadC_C172A,C208D,C272K	cadC_C172A,C208D,C272K in pET16b	This work
pBAD33	Expression vector, Cm^r	[48]
pBAD33-lysP	lysP in pBAD33	[11]

Site-directed mutants are designated as follows: The one letter code is used, followed by a number indicating the position of the amino acid in wild-type CadC.
The sequence is followed by a second letter denoting the amino acid replacement at this position.

ISSN: 1471-2180