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Table 1 Strains and plasmids used in this study

From: Contribution of SecDF to Staphylococcus aureusresistance and expression of virulence factors

Strain Relevant genotype or phenotype Ref. or source
S. aureus   
   Newman Clinical isolate (ATCC 25904), rsbU+ [64]
   RN4220 NCTC8325-4 r- m+ [65]
   CQ33 NewmanΔsa2056 This study
   CQ39 Newman pME2, Tcr, Mcr This study
   CQ65 NewmanΔsa2339 This study
   CQ66 NewmanΔsecDF This study
   CQ69 NewmanΔsecDF pME2, Tcr, Mcr This study
   CQ85 Newman pCN34, Kmr This study
   CQ86 Newman pCN34 pME2, Kmr, Tcr, Mcr This study
   CQ87 NewmanΔsecDF pCN34, Kmr This study
   CQ88 NewmanΔsecDF pCN34 pME2, Kmr, Tcr, Mcr This study
   CQ89 NewmanΔsecDF pCQ27, Kmr This study
   CQ90 NewmanΔsecDF pCQ27 pME2, Kmr, Tcr, Mcr This study
E. coli   
   DH5α Cloning strain, [F-Φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-] Invitrogen
Plasmid Relevant genotype or phenotype Reference or source
   pCN34 S. aureus-E. coli shuttle vector, pT181-cop-wt repC aphA-3 ColE1 Kmr [56]
   pCQ27 pCN34 derivative carrying secDF and its promoter (Newman), Kmr This study
   pCQ30 pKOR1 derivative carrying 1 kb fragments of the region up- and downstream of sa2056 amplified from Newman, ligated together with EcoRI and recombined at the attP sites, Apr, Cmr This study
   pCQ31 pKOR1 derivative carrying 1 kb fragments of the region up- and downstream of sa2339 amplified from Newman, ligated together with HindIII and recombined at the attP sites, Apr, Cmr This study
   pCQ32 pKOR1 derivative carrying 1 kb fragments of the region up- and downstream of secDF amplified from Newman, ligated together with HindIII and recombined at the attP sites, Apr, Cmr This study
   pKOR1 E. coli-S. aureus shuttle vector used to create markerless deletions; repF(Ts) cat attP ccdB ori ColE1 bla P xyl /tetO secY570, Apr, Cmr [23]
   pME2 pBUS1 derivative carrying mecA and its promoter (COLn), Tcr, Mcr [28]
  1. Abbreviations are as follows: Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Kmr, kanamycin resistant; Mcr methicillin resistant; Tcr, tetracycline resistant.