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Table 4 Comparison of the codon usage in the arcA gene between E. coli K12 MG1655 and BL21 (DE3) based on Chen & Texada, [66].

From: Effect of iclR and arcA knockouts on biomass formation and metabolic fluxes in Escherichia coli K12 and its implications on understanding the metabolism of Escherichia coliBL21 (DE3)

AA Strain Codon Frequency tRNA content
L MG1655 CUG 54.1 1
  BL21 CUA 2.97 Minor
S MG1655 UCU 10.47 0.25
  BL21 UCC 9.43 Minor
P MG1655 CCA 8.12 Major
  BL21 CCG 23.91 Major
I MG1655 AUC 26.97 1
  BL21 AUU 27.27 1
C MG1655 UGU 4.8 Minor
  BL21 UGC 6.07 Minor
  1. Each codon is expressed as the frequency per 1000 codons. The content is the relative amount to that of tRNALeu1(CUG), which is normalized to 1 and approximately in the order of 104 molecules per cell for normally growing E. coli cells