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Table 4 Comparison of the codon usage in the arcA gene between E. coli K12 MG1655 and BL21 (DE3) based on Chen & Texada, [66].

From: Effect of iclR and arcA knockouts on biomass formation and metabolic fluxes in Escherichia coli K12 and its implications on understanding the metabolism of Escherichia coliBL21 (DE3)

AA

Strain

Codon

Frequency

tRNA content

L

MG1655

CUG

54.1

1

 

BL21

CUA

2.97

Minor

S

MG1655

UCU

10.47

0.25

 

BL21

UCC

9.43

Minor

P

MG1655

CCA

8.12

Major

 

BL21

CCG

23.91

Major

I

MG1655

AUC

26.97

1

 

BL21

AUU

27.27

1

C

MG1655

UGU

4.8

Minor

 

BL21

UGC

6.07

Minor

  1. Each codon is expressed as the frequency per 1000 codons. The content is the relative amount to that of tRNALeu1(CUG), which is normalized to 1 and approximately in the order of 104 molecules per cell for normally growing E. coli cells