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Table 2 Relative activities of malate synthase and isocitrate lyase under glucose abundant (batch) and limiting (chemostat) conditions.

From: Effect of iclR and arcA knockouts on biomass formation and metabolic fluxes in Escherichia coli K12 and its implications on understanding the metabolism of Escherichia coliBL21 (DE3)

 

Isocitrate lyase activity

Malate synthase activity

Strain

Batch

Chemostat

Batch

Chemostat

MG1655

1.00 ± 0.10

10.13 ± 1.43

1.00 ± 0.19

0.11 ± 0.03

MG1655 ΔarcA

0.33 ± 0.04

32.47 ± 3.61

0.36 ± 0.07

2.13 ± 0.39

MG1655 ΔiclR

5.69 ± 0.57

26.96 ± 3.06

1.38 ± 0.27

0.24 ± 0.04

MG1655 ΔarcAΔiclR

6.39 ± 0.64

26.52 ± 2.78

0.48 ± 0.08

2.92 ± 0.52

  1. Arbitrarily, all enzyme activities are scaled to the wild type activities under glucose abundant conditions.
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BMC Microbiology

ISSN: 1471-2180

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