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Table 1 E. faecalis strains and plasmids used in this study.

From: Deletion of the glycosyltransferase bgsB of Enterococcus faecalisleads to a complete loss of glycolipids from the cell membrane and to impaired biofilm formation

strain or plasmid characterization reference
strains   
E. faecalis 12030 Clinical isolate, strong biofilm producer [33]
E. faecalis ATCC 29212 Reference strain  
E. faecalis 12030ΔbgsA (EF2891) bsgA mutant [5]
E. faecalis 12030ΔbgsB bgsB deletion mutant This study
E. faecalis 12030ΔbgsB_rec. Reconstituted mutant This study
Escherichia coli DH5α Gram-negative cloning host  
Escherichia coli TOP10 Gram-negative cloning host Invitrogen
plasmids   
pCASPER Gram-positive, temperature-sensitive mutagenesis vector [34]
pCRII-TOPO Gram-negative cloning vector Invitrogen
pCASPER/ΔbgsB   This study
pMAD/bsgB   This study
pMAD oripE194ts, EmR, AmpR, bgaB [35]