Deletion of the glycosyltransferase bgsB of Enterococcus faecalisleads to a complete loss of glycolipids from the cell membrane and to impaired biofilm formation

Table 1 E. faecalis strains and plasmids used in this study.

strain or plasmid	characterization	reference
strains
E. faecalis 12030	Clinical isolate, strong biofilm producer	[33]
E. faecalis ATCC 29212	Reference strain
E. faecalis 12030ΔbgsA	(EF2891) bsgA mutant	[5]
E. faecalis 12030ΔbgsB	bgsB deletion mutant	This study
E. faecalis 12030ΔbgsB_rec.	Reconstituted mutant	This study
Escherichia coli DH5α	Gram-negative cloning host
Escherichia coli TOP10	Gram-negative cloning host	Invitrogen
plasmids
pCASPER	Gram-positive, temperature-sensitive mutagenesis vector	[34]
pCRII-TOPO	Gram-negative cloning vector	Invitrogen
pCASPER/ΔbgsB		This study
pMAD/bsgB		This study
pMAD	oripE194^ts, Em^R, Amp^R, bgaB	[35]

ISSN: 1471-2180