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Figure 4 | BMC Microbiology

Figure 4

From: Deletion of the glycosyltransferase bgsB of Enterococcus faecalisleads to a complete loss of glycolipids from the cell membrane and to impaired biofilm formation

Figure 4

Comparison of 1H-NMR spectra of LTA isolated from E. faecalis 12030 wt, 12030Δ bgsB , and 12030Δ bgsA. Comparison of integration values of fatty acid (FA) signals (-CH2- and -CH3) as an internal reference and anomeric proton signal of glucose (H1 Glc A and H1 Glc B) indicated that the glycerolphosphate polymer of LTA from 12030ΔbgsB and 12030ΔbgsA contains approximately four times more kojibiose. Comparison of the resonance signal of total alanine (-CH3 Ala) and fatty acid signals (-CH2- (FA) and -CH3 (FA)) revealed that LTA extracted from either mutant also contains more alanine residues. Gro - glycerol.

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BMC Microbiology

ISSN: 1471-2180

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