Figure 3From: Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteinsDetection of RD2 transfer from donor strain MGAS6180 ( emm28 ) to recipient strain MGAS10750 ( emm4 ). Amplicons 1-12 generated by PCR tiling across the RD2 element. A. transconjugant; * denote amplicons encompassing deleted M28_Spy1325-1326 region that is replaced by spectinomycin resistance cassette; B. control with chromosomal DNA isolated from strain MGAS6180. M - 1 kb ladder (Invitrogen)Back to article page