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Table 3 Impact of a 3.6%-Oxgall exposure on specific proteomic patterns putatively related to bile tolerance

From: Comparative proteomic analysis of Lactobacillus plantarumfor the identification of key proteins in bile tolerance

Functional category

Protein

Stressa)

Geneb)

Spot number

Normalized volume with 3.6% Oxgallc)

Variation factor: bile vs. standard conditionsd)

     

LC 56

LC 804

299 V

LC 56

LC 804

299 V

Translation, ribosomal structure and biogenesis

Ribosomal protein S30EA

B [14]

lp_0737

62

0.049 ± 0.004

-

-

-3.2

-

-

Posttranslational modification, protein turnover, chaperones

α-Small heat shock protein

O [55]

lp_0129 (hsp1)

1

0.952 ± 0.059

1.008 ± 0.190

0.597 ± 0.082

34

11.4

2.1

   

lp_3352 (hsp3)

4

-

1.172 ± 0.159

0.744 ± 0.171

-

1.7

2.2

 

Chaperonin GroEL

B [14]

lp_0728 (groEL)

76

27.427 ± 1.216

14.137 ± 0.142

11.931 ± 0.715

3.7

1.9

-1.1*

 

ATP-dependent Clp protease

D [56]

lp_0786 (clpP)

77

-

0.360 ± 0.072

0.282 ± 0.020

-

2.0

1.7

Energy production and conversion

F0F1 ATP synthase subunit delta

B [44]

lp_2367

90

-

0.243 ± 0.051

0.110 ± 0.012

-

4.3

1.2*

 

Glutathione reductase

O [57]

lp_3267 (gshR4)

19

0.179 ± 0.023

0.011 ± 0.001

0.210 ± 0.008

-1.8

-1.8

-1.3

   

lp_0369 (gshR1)

24

-

0.314 ± 0.025

0.148 ± 0.009

-

1.1*

-1.6

Carbohydrate transport and metabolism

Glucose-6-phosphate 1-dehydrogenase

B [14], O [58]

lp_2681 (gpd)

26

-

0.098 ± 0.005

0.116 ± 0.025

-

-1.2*

-1.4

Amino-acid transport and metabolism

Glycine/betaine/carnitine/choline ABC transporter

B [48], S [58]

lp_1607 (opuA)

18

-

0.034 ± 0.003

0.081 ± 0.007

-

-1.6

1.5

Nucleotide transport and metabolism

Bifunctional GMP synthase/glutamine amidotransferase protein

A [35]

lp_0914 (guaA)

80

0.039 ± 0.003

0.104 ± 0.009

0.209 ± 0.016

-7.6

-1.8

12.5

Inorganic ion transport and metabolism

Stress-induced DNA binding protein

O [59]

lp_3128 (dps)

34

0.278 ± 0.026

0.074 ± 0.003

1.212 ± 0.124

2.6

2.0

1.0*

    

41

0.957 ± 0.077

-

-

2.5

-

-

Cell wall/membrane/envelope biogenesis

Bile salt hydrolase

B [49]

lp_3536 (bsh1)

11

-

-

0.061 ± 0.008

-

-

-2.6

 

dTDP-4-Dehydro-rhamnose 3,5-epimerase

O, D [60]

lp_1188 (rfbC)

42

0.151 ± 0.010

-

-

1.1*

-

-

 

Cyclopropane-fatty-acyl-phospholipid synthase

A [42, 43]

lp_3174 (cfa2)

64

0.0312 ± 0.002

0.069 ± 0.007

-

-6.9

-2.5

-

    

72

-

0.046 ± 0.004

0.052 ± 0.001

-

-2.6

1.0*

  1. a) Reported implication of the protein in bile (B), oxidative (O), acid (A), detergent (D) and/or salt (S) stress tolerance with the corresponding references.
  2. b) Gene accession number in the NCBI database for L. plantarum WCFS1 with the general symbol of the gene in brackets.
  3. c) Normalized relative volumes, expressed as a percentage of total valid spots. Values are means ± standard deviations; n ≥ 3 for each strain. -, not detected.
  4. d) r = volume with bile salt/volume without bile salt for the considered strain. When r > 1, variation factor = r. When r < 1, variation factor = -1/r.
  5. * means of volumes with and without Oxgall are not statistically different (Student's t test for paired samples, p < 0.05).
  6. These patterns gather differentially expressed proteins in standard growth conditions among L. plantarum LC 56, LC 804, and 299 V that have previously been reported to be involved in BOADS stress tolerance based on dedicated mutant analysis. The impact of exposure to bile is assessed through protein expression comparison for early stationary cells cultured with and without Oxgall, using normalized relative volumes. Normalized volumes in standard conditions are listed in Additional file 1.
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BMC Microbiology

ISSN: 1471-2180

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