Figure 3
Kinetics of ppGpp accumulation in ECOR strains starved for carbon or amino acid. 32P-labelled cultures of exponentially-growing cells were treated with 2% α-MG (to induce carbon starvation) or 1 mg/ml SH (to induce amino acid starvation). Samples were withdrawn at time intervals and assayed for ppGpp. Values represent the level of ppGpp relative to GTP + ppGpp.