Figure 3
From: Characterization of a ranavirus inhibitor of the antiviral protein kinase PKR
vIF2α does not complement eIF2α function in yeast. Plasmids expressing VACV K3L (pC140) or RCV-Z vIF2α (pC3853) under the control of a yeast GAL-CYC1 hybrid promoter, or yeast eIF2α (p919) under the control of its native promoter, or the vector pEMBLyex4, were introduced into the temperature-sensitive eIF2α (sui2-1, TD304-10B) mutant strain. The indicated transformants were streaked on SC-Gal medium, where eIF2α expression was maintained and the viral protein expression was induced, and incubated at the indicated temperatures. Results shown are representative of 4 independent transformants for each plasmid.