Molecular and virulence characteristics of an outer membrane-associated RTX exoprotein in Pasteurella pneumotropica

Table 1 Bacterial strains and plasmids used in this study

Strain or plasmid	Description	Source or reference
Strains
Pasteurella pneumotropica
ATCC 35149	Type strain, biotype Jawetz, isolated from mouse lung	ATCC^a [50]
ATCC 12555	Biotype Heyl, isolated from mouse	ATCC [51]
CCUG 26450	Biotype Jawetz, isolated from gerbil	CCUG^b
CCUG 26451	Biotype Jawetz, isolated from hamster	CCUG
CCUG 26453	Biotype Heyl, isolated from bird	CCUG
CCUG 36632	Biotype unknown, isolated from murine nose	CCUG
Escherichia coli
DH5α	Cloning strain	Stratagene
TOP10	Cloning strain	Invitrogen
BL21-AI	Protein expression strain	Invitrogen
TMU0812	BL21-AI ΔhlyE::Km^r	[13]
Plasmids
pTAC-1	Cloning vector, Ap^r	Biodynamics Laboratory
pENTR/SD/D-TOPO	Entry vector, Km^r	Invitrogen
pBAD-DEST49	Protein expression vector, N-terminal fusions to thioredoxin tag and C-terminal fusions to six-Histidine tag, Ap^r	Invitrogen
pET300/NT-DEST	Protein expression vector, N-terminal fusions to six-Histidine tag, Ap^r	Invitrogen
pTAC-PX3	0.5-kb pnxIIIA PCR fragment	This study
pBAD-Pnx3A	Entire pnxIIIA gene cloned into pBAD-DEST49	This study
pBAD-Pnx3A₂₀₉	1.3-kb sequence of repeat 1 deleted from pBAD-Pnx3A	This study
pBAD-Pnx3A₁₉₇	1.7-kb sequence of repeats 2 and 3 deleted from pBAD-Pnx3A	This study
pBAD-Pnx3A₁₅₁	3.0-kb sequence of repeats 1, 2, and 3 deleted from pBAD-Pnx3A	This study
pET-Pnx3E	Entire pnxIIIE gene cloned into pET300/NT-DEST	This study

ISSN: 1471-2180