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Table 1 Bacterial strains and plasmids used in this study

From: Molecular and virulence characteristics of an outer membrane-associated RTX exoprotein in Pasteurella pneumotropica

Strain or plasmid Description Source or reference
Strains   
   Pasteurella pneumotropica   
ATCC 35149 Type strain, biotype Jawetz, isolated from mouse lung ATCCa [50]
ATCC 12555 Biotype Heyl, isolated from mouse ATCC [51]
CCUG 26450 Biotype Jawetz, isolated from gerbil CCUGb
CCUG 26451 Biotype Jawetz, isolated from hamster CCUG
CCUG 26453 Biotype Heyl, isolated from bird CCUG
CCUG 36632 Biotype unknown, isolated from murine nose CCUG
   Escherichia coli   
DH5α Cloning strain Stratagene
TOP10 Cloning strain Invitrogen
BL21-AI Protein expression strain Invitrogen
TMU0812 BL21-AI ΔhlyE::Kmr [13]
Plasmids   
   pTAC-1 Cloning vector, Apr Biodynamics Laboratory
   pENTR/SD/D-TOPO Entry vector, Kmr Invitrogen
   pBAD-DEST49 Protein expression vector, N-terminal fusions to thioredoxin tag and C-terminal fusions to six-Histidine tag, Apr Invitrogen
   pET300/NT-DEST Protein expression vector, N-terminal fusions to six-Histidine tag, Apr Invitrogen
   pTAC-PX3 0.5-kb pnxIIIA PCR fragment This study
   pBAD-Pnx3A Entire pnxIIIA gene cloned into pBAD-DEST49 This study
   pBAD-Pnx3A209 1.3-kb sequence of repeat 1 deleted from pBAD-Pnx3A This study
   pBAD-Pnx3A197 1.7-kb sequence of repeats 2 and 3 deleted from pBAD-Pnx3A This study
   pBAD-Pnx3A151 3.0-kb sequence of repeats 1, 2, and 3 deleted from pBAD-Pnx3A This study
   pET-Pnx3E Entire pnxIIIE gene cloned into pET300/NT-DEST This study
  1. aAmerican Type Culture Collection
  2. bCulture Collection of the University of Göteborg