Table 1 Purification of A. pernix cytochrome c553.
Steps | Total Protein (mg) | c-type cytochrome | |
|---|---|---|---|
Total (nmol) | Specific (nmol mg-1) | ||
Membranes | 589 | 463 | 0.787 |
DDM extract | 494 | 344 | 0.697 |
DEAE-Toyopearl | 27.1 | 105 | 3.88 |
Q-Sepharose | 21.1 | 30.8 | 1.46 |
Hydroxyapatite | 2.00 | 15.1 | 7.55 |