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Table 1 Virulence characteristics of K. pneumoniae 1112, KPG6, and 1084.

From: Assessment of hypermucoviscosity as a virulence factor for experimental Klebsiella pneumoniaeinfections: comparative virulence analysis with hypermucoviscosity-negative strain

Characteristics K. pneumoniaestrains
  1112 KPG6 1084
Hypermucoviscosity Positive Negative Negative
Serum killinga Resistant Sensitive Resistant
Oral LD50b 6.9 × 106 > 109 9 × 106
Intracellular survival (%) in Raw264.7 cellc 60.29 ± 5.04 46.77 ± 1.61 57.82 ± 2.42
  1. Note. aIf the bacteria examined showed a reduction of 2 log10 in the surviving counts after serum incubation, this strain was defined as serum-sensitive. If the viability of a strain remain > 90% after 30 min-treatment, the strain was defined as serum-resistant. bFive 8 wk old male BALB/c mice of a group were orally inoculated with bacterial culture of a particular K. pneumoniae strain in 10-fold steps graded doses. The 50% lethal doses, based on the number of survivors after one week, were calculated by the method of Reed and Muench [29] expressed as colony forming units (CFU). cA particular K. pneumoniae strain was used to infect Raw264.7 cells with m.o.i. = 100. After stringent washes, the number of adherent and intracellular K. pneumoniae was determined before and after gentamicin treatment. The intracellular survival rate was calculated as 100% × (number of intracellular bacteria after gentamicin treated for 3 h/number of adherent bacteria before gentamicin treatment).