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Figure 3 | BMC Microbiology

Figure 3

From: Hepatitis B virus X protein impedes the DNA repair via its association with transcription factor, TFIIH

Figure 3

Reduced interaction of HBX mutants with RAD3 (ERCC2 homolog) and SSL2 (ERCC3 homolog) components of yeast TFIIH. (A) RAD3 was in vitro translated in the presence of 35S methionine and allowed to interact with GST (lane 1) or GST-X (lane 2), GST-XAsp113 (lane 3), GST-X Asp 118, (lane 4) GST-XGlu120 (lane 5), GST-X Glu121 (lane 6), GST-X Glu 124 (lane 7), GST-XGlu 125 (lane 8) and GST-X Glu 120/21 (lane 9).(B) SSL2 was synthesized in vitro and labeled with 35S methionine and allowed to interact with GST (lane 1) or GST-X (lane 2), GST-XAsp113 (lane 3), GST-X Asp 118, (lane 4) GST-XGlu120 (lane 5), GST-X Glu121 (lane 6), GST-X Glu 124 (lane 7), GST-XGlu 125 (lane 8), and GST-X Glu 120/21 (lane 9).

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