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Table 1 Effect of heat treatment or the presence of polymyxin B on cytokine secretion by PMA-differentiated U937 macrophages stimulated with the recombinant SspA (33 μg/ml) of S. suis.

From: The SspA subtilisin-like protease of Streptococcus suis triggers a pro-inflammatory response in macrophages through a non-proteolytic mechanism

Conditions Amount secreted (pg/ml)
  CCL5 IL-6 CXCL8
Control (no stimulation) 2081 ± 14 100 ± 1 3170 ± 9
Recombinant SspA of S. suis 2370 ± 61* 1922 ± 31* 108557 ± 620*
Heat-inactivated recombinant SspA of S. suis 72409 ± 848* 2111 ± 71* 102287 ± 1062*
Recombinant SspA of S. suis + polymyxin B 2081 ± 32 2099 ± 254* 107446 ± 590*
  1. The data are the means ± SD of triplicate assays for three separate experiments. Asterisks indicate a significant difference in comparison with the unstimulated control at P < 0.01.