Bacillus anthracis spore interactions with mammalian cells: Relationship between germination state and the outcome of in vitro

Table 2 Germination and outgrowth of B. anthracis spores as a function of FBS concentration^a.

^a Three independent experiments were performed with three different spore preparations, each conducted in triplicate.
^b Spores prepared from B. anthracis Sterne 7702 were incubated in DMEM.
^c Indicates the concentration of FBS used in the DMEM.
^d Spores were scored positive (+) for germination if the OD_{600 nm} of the suspended spores decreased by more than 5% after 30 min incubation in the indicated medium.
^e Using DIC microscopy, spores were scored positive (+) for outgrowth if the spores bodies were visibly larger at 1 h, and had developed into vegetative bacteria by 4 h.

ISSN: 1471-2180