Quinolone resistance in Escherichia coli from Accra, Ghana

BMC Microbiology

Table 2 Oligonucleotide primers used in this study

Target gene	Primer	Primer Sequence	Purpose	Reference
gyrA	gyrA12004	TGC CAG ATG TCC GAG AT	gyrA QRDR amplification	[12]
	gyrA11753	GTA TAA CGC ATT GCC GC
parC	EC-PAR-A	CTG AAT GCC AGC GCC AAA TT	parC QRDR amplification	[43]
	EC-PAR-B	GCG AAC GAT TTC GGA TCG TC
qnrA	qnrA-1A	TTC AGC AAG ATT TCT CA	qnrA detection	[42]
	qnrA-1B	GGC AGC ACT ATT ACT CCC AA
qnrB	qnrB-CS-1A	CCT GAG CGG CAC TGA ATT TAT	qnrB detection	[42]
	qnrB-CS-1B	GTT TGC TGC TCG CCA GTC GA
qnrS	qnrS-1A	CAA TCA TAC ATA TCG GCA CC	qnrS detection	[42]
	qnr-1B	TCA GGA TAA ACA ACA ATA CCC
qepA	qepA-F	GCAGGTC CAGCAGCGGGTAG	qepA detection	[41]
	qepA-R	CTTCCTGCCCGAGTATC GTG
adk	adkF	ATTCTGCTTGGCGCTCCGGG	MLST	[19]
	adkR	CCGTCAACTTTCGCGTATTT
fumC	fumCF	TCACAGGTCGCCAGCGCTTC	MLST	[19]
	fumCR	GTACGCAGCGAAAAAGATTC
gyrB	gyrBF	TCGGCGACACGGATGACGGC	MLST	[19]
	gyrBR	ATCAGGCCTTCACGCGCATC
icd	icdF	ATGGAAAGTAAAGTAGTTGTTCCGGCACA	MLST	[19]
	icdR	GGACGCAGCAGGATCTGTT
mdh	mdhF	ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG	MLST	[19]
	mdhR	TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT
purA	purAF	CGCGCTGATGAAAGAGATGA	MLST	[19]
	purAR	CATACGGTAAGCCACGCAGA
recA	recAF	CGCATTCGCTTTACCCTGACC	MLST	[19]
	recAR	TCGTCGAAATCTACGGACCGGA

MLST - multi-locus sequence typing; QRDR - quinolone-resistance determining region

ISSN: 1471-2180