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Table 3 List of plasmids used in this study.

From: Biochemical and physiological characterization of the GTP-binding protein Obg of Mycobacterium tuberculosis

Plasmid

Description

Reference/source

pCR2.1

oriColE1, lacZα, Plac, aph, AmpR

Invitrogen

pMV261

oriE, oriM, Phsp60, aph

Stover et al, 1991

pMVOBG

pMV261-Rv2440c full orf

This study

pET16b

oriE, lacI, PT7, AmpR

Novagen

pTBOBGE

pET16B-Rv2440c full orf

This study

pGADT7

oriColE1, ori2 μ, LEU1, PADH1::GAL4' activator domain::MCS AmpR

Clontech

pGBKT7

oriColE1, ori2 μ, TRP1, PADH1::GAL4' binding domain::MCS KmR

Clontech

pGADT7-T

SV40 large T-antigen(84-708) in pGADT7

Clontech

pGBKT7-53

Murine p53(72-390) in pGBKT7

Clontech

pGBKT7-Lam

Human lamin C(66-230) in pGBKT7

Clontech

pGA2853c

pGADT7-Rv2853c full orf

This study

pGB3286c

pGBKT7-Rv3286c full orf

Parida et al, 2005

pGA3287c

pGADT7-Rv3287c full orf

Parida et al, 2005

pGB2440c

pGBKT7-Rv2440c full orf

This study