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Table 1 Interaction of Obg with stress related proteins in the yeast two-hybrid system.

From: Biochemical and physiological characterization of the GTP-binding protein Obg of Mycobacterium tuberculosis

 

*Plasmids

SD Minimal Medium

Mel-l (α-gal) in

SD plates

Mel-1 (α-gal) in

SD broth**

  

-Leu/

-Trp

-His/

-Leu/-Trp

-Ade/-His/

-Leu/-Trp

  

1.

pGADT7-T

+

+

+

+++

3.512 ± 0.709

 

pGBKT7-53

     

2.

pGADT7-T

+

-

-

-

-

 

pGBKT7-Lam

     

3.

pGA3287c

+

+

+

++

2.367 ± 0.354

 

pGB3286c

     

4.

pGA3287c

+

+

+

++

2.172 ± 0.448

 

pGB2440c

     

5.

pGA2853c

+

-

-

-

-

 

pGB2440c

     
  1. Abbreviations. SD, synthetic drop out medium; Ade, Adenine; His, Histidine; Leu, Leucine; Trp, Tryptophan; Mel-1, α-galactosidase.
  2. *1-5 indicate plasmids cotransformed into yeast strain AH109 (HIS3, ADE2, MEL1) (Clontech).
  3. **α-galactosidase (Mel-1) expressed as Mean ± SD milli units/A600. Plasmids are described in Table 3.