Single nucleotide polymorphism in the genes of mce1 and mce4 operons of Mycobacterium tuberculosis: analysis of clinical isolates and standard reference strains

Table 3 Codon usage in mce1 and mce4 operons

Operon	Gene name (Accession Number)	Wild type codon	Polymorphic codon
*mce1* operon	mce1A [Rv0169]	TAC	TAT
	yrbE4A [Rv3501c]	GCG ATC	GCT ATA
*mce4*	yrbE4B [Rv3500c]	ATC CCC	ATT CCT
operon	mce4A [Rv3499c]	TTC	TTT
	lprN [Rv3495c]	GCC	GCT
	mce4F [Rv3494c]	ACC	ACA

The codon usage in the polymorphic regions is shown here. The synonymous changes in the nucleotide sequence, when analysed bioinformatically through Gene Runner software version 3.05 (Hastings Software, Inc.) predicts the usage of less preferred codon which could reflect upon the expression efficiency of the protein encoded by the gene.
Nucleotide highlighted in bold indicates the altered nucleotide.

ISSN: 1471-2180