Figure 7
TbrPPX1 does not exhibit cyclic nucleotide phosphodiesterase activity. Panel A: PDE activity of immunoprecipitates from procyclic cells expressing c-Myc-tagged TbrPPX1 and TbrPDEB2, respectively, and from wild type procyclics. The results of two independent experiments are given for each. Panel B: Western blotting demonstrating that the respective proteins are expressed and present in the lysates used for immunoprecipitation. Panel C: Complementation of PDE-deficient S. cerevisiae. First row: strain expressing T. cruzi PDEC (positive control); rows 2 - 6: clones expressing TbrPPX1; row 7: strain carrying the empty vector (negative control). Each row from left to right: serial 10-fold dilutions, 5 μl spotted.