Figure 4
From: pBAM1: an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes
Subcellular localization of high-fluorescence GFP fusions generated by mutagenesis of P. putida with mini-Tn 5 GFPKm. Cultures of the cells under examination were grown until stationary phase in LB medium and prepared for epifluorescence microscopy as explained in Materials and Methods. The upper panel shows examples of GFP fused to cytosolic proteins, as indicated, whereas in lower panel contains GFP fusions in three different membrane-associated proteins. Table S4 of Additional File 1 provides more details of the GFP fusions generated.