Structural organization of standard mini-transposon modules. (A) Mini-Tn5 Km. Details of relevant restriction enzymes within the module are shown. The fusion of ME-I and ME-O sequences with the plasmid DNA backbone generated PvuII restriction sites that bracket the mobile segment. The red arrow indicates the position of the promoter of the Km resistance gene. MCS: multiple-cloning-site. (B) mini-Tn5GFPKm. Schematic representation of the main features of this version of the mini-transposon engineered in the pBAM1 backbone, containing the GFP gene lacking leading sequences and thus able to produce protein fusions upon chromosomal insertions in the right direction and frame. The Km resistance cassette is identical to that of the mini-Tn5Km of pBAM1.