Table 2 Physiological characteristics of N. europae a wild type and fur:kanP mutant grown under Fe-replete (10 μM) and Fe-limited (0.2 μM) conditions*

Heme c content in cell's

soluble fraction

Heme c (nmol/ml culture)

0.85 ± 0.02

0.38 ± 0.05

0.48 ± 0.02

0.21 ± 0.04

Heme c (nmol/mg protein)

7.77 ± 0.23

4.04 ± 0.53

5.67 ± 0.31

5.04 ± 0.91

Whole Cell Fe content

Fe (nmol/ml culture)

1.36 ± 0.15

0.15 ± 0.01

2.04 ± 0.09

0.11 ± 0.01

Fe (nmol/mg protein)

90.4 ± 6.0

26.4 ± 2.0

136.2 ± 14.0

24.9 ± 3.0

Cellular Fe concentration (mM)

8.27 ± 0.94

1.99 ± 0.13

12.4 ± 0.6

1.98 ± 0.18

Whole cell enzyme-catalyzed activity

NH₄⁺-dependent O₂ consumption (nmol/(min × OD_{600 nm})

94.5 ± 4.1

38.1 ± 6.0

88.2 ± 2.5

21.7 ± 0.6

NH₄⁺-dependent O₂ consumption (nmol/(min × mg protein)

1500 ± 63

779 ± 17

1446 ± 40

680 ± 18

NH₂OH-dependent O₂ consumption (nmol/(min × OD_{600 nm})

25.9 ± 0.2

10.9 ± 2.4

25.7 ± 4.8

4.6 ± 0.2

NH₂OH-dependent O₂ consumption (nmol/(min × mg protein)

412 ± 3.0

222 ± 5.0

421 ± 2.0

146 ± 6.0