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Table 3 Oligonucleotides used

From: Role of ZnuABC and ZinT in Escherichia coliO157:H7 zinc acquisition and interaction with epithelial cells

Primer

Sequence(5'-3')

ZinT-HP1

TTTAGGTGTCTTTATTGTTAGCGCTCCTGCCTTTTCGCATTGTAGGCTGGAGCTGCTTCG

ZinT-HP2

CCACTTCTTCGCTACTCAACTGATATGGATAATACGTTGGCCCATATGAATATCCTCCTTAG

ZnuA-HP1

ATTATTCGCCGCTCTCTGGGGCGGTGCAACACAGGCCGCTGTAGGCTGGAGCTGCTTCG

ZnuA-HP2

CCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGCATATGAATATCCTCCTTAG

Zur-HP1

AAATCTGCGCGCAGCGTAATGTGCGCCTGACCCCACAGCTGTAGGCTGGAGCTGCTTCG

Zur-HP2

GCACAGAGTGATCATGCTGGCACTGTTCAGGATGACGACACGCCATATGAATATCCTCCTTAG

EtpC-HP1

ATAAAAGATATCGTACTTAAAATGCTGACGCCAAACCGGCTGTAGGCTGGAGCTGCTTCG

EtpC-HP2

CAGCAATAAATGCATCATATAACTGACCATCACGCTCGACCATATGAATATCCTCCTTAG

EtpD-HP1

TTTATGGTTTTGCTGTGGTCGATATGCACAACGGTATACTTGTAGGCTGGAGCTGCTTCG

EtpD-HP2

GCATAAAACGCAGCAATCGCCGCTTTCACCTTCCGGAAAGCATATGAATATCCTCCTTAG

ZinT-3xFLAGF

GTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATGACTACAAAGACCATGACGG

ZinT-3xFLAGR

CTTTCTCTGTTGGCCGTATTGTGTATGGAATCCGTTATTGGCATATGAATATCCTCCTTAG

ZnuA-3xFLAGF

TCAATTAGCCAACCAGTATGCGAGCTGCCTGAAAGGAGATGACTACAAAGACCATGACGG

ZnuA-3xFLAGR

TGACAATTGGCGTGGCATCGCGGTGATAAACATAGGGCCGCATATGAATATCCTCCTTAG

ZnuAO157-Pst-F

AACTGCAGTGTCGACTTACCTGCG

ZnuAO157-Xba-R

GCTCTAGATTATTAAACGCCAGGGCGA

ZnuA E coliKpn-F

GGGGTACCGTTACATAAAAAAACGCTTC

ZnuA E coliXba-R

GCTCTAGATTAATCTCCTTTCAGGCAG

Clo-Inta

CTGGATATACCACCGTTGAT

Stop Clo-Inta

CACTCATCGCAGTACTGTT

Kan-Fa

TGAACAAGATGGATTGCACG

Kan-Ra

AAGAACTCGTCAAGAAGGC

  1. P1, P2 and 3xFLAG homolog sequences are underlined
  2. a primers used for screening of mutant clones or in Southern experiments