Figure 8

Influence of extracellular ZinT on z nu A expression. RG-F120 (ΔzinT::cat znuA::3xFLAG-kan) strain was grown in LB medium (lanes 2, 3, 5 and 6) or LB supplemented with 0.5 mM EDTA (lanes 4 and 7) in presence of 25 μg (lanes 2, 3 and 4) or 0.25 μg (lanes 5, 6 and 7) extracellular ZinT. The extracts, analyzed by Western blot, were prepared after a 4 h growth (lanes 3, 4, 6 and 7), or immediately after the addition of extracellular ZinT (lanes 2 and 5), as negative control. 25 μg of extracellular ZinT was loaded in lane 1 as positive control.