Figure 7
Extracellular ZinT accumulation. Panel A: RG-F116 (zinT::3xFLAG-kan) strain was grown in LB medium supplemented with 0.5 mM EDTA (lanes 1 and 3) or with 0.25 mM CdSO4 (lanes 2 and 4). After 4 h of growth, total (lanes 1 and 2) or extracellular extracts (lanes 3 and 4) were loaded on SDS-PAGE and analyzed by Western blot. Panel B: RG-F116 (lanes 1 and 2) and RG-F121 (ΔznuA::cat zinT::3xFLAG-kan) strains (lanes 3, 4, 5 and 6) were grown in modM9 (lanes 1, 2, 3 and 4) or supplemented with 5 μM of ZnSO4 (lanes 5 and 6). After 6 h of growth, total (lanes 1, 3 and 5) or extracellular extracts (lanes 2, 4 and 6) were loaded on SDS-PAGE and analyzed by Western blot.