Figure 4
Zinc-dependent ZinT and ZnuA accumulation in E. coli O157:H7 and S. enterica strains. RG-F116 (zinT::3xFLAG-kan), RG-F117 (znuA::3xFLAG-kan) E. coli O157:H7 strains or PP134 (zinT::3xFLAG-kan) and SA140 (znuA::3xFLAG- kan ilvI::Tn10dTac-cat:: 3xFLAG- kan) S. enterica strains were grown for 16 h in modM9 supplemented or not with various concentrations of ZnSO4, as indicated. The extracts were analyzed by Western blot. In SA140 strain the chloramphenicol acetyltransferase (CAT) was used as an internal standard.