Figure 3From: Role of ZnuABC and ZinT in Escherichia coliO157:H7 zinc acquisition and interaction with epithelial cellsInfluence of metals on ZinT and ZnuA accumulation. RG-F116 (zinT::3xFLAG-kan) and RG-F117 (znuA::3xFLAG-kan) strains were grown for 16 h in modM9 (lanes 1 and 6) in presence of ZnSO4 (lanes 2 and 7), FeSO4 (lanes 3 and 8), CuSO4 (lanes 4 and 9) or MnCl2 (lanes 5 and 10). Metal concentration was 5 μM. The extracts were analyzed by Western blot.Back to article page