Figure 4
Efflux of EtBr by M. smegmatis mc2155, XZL1675 (Δ lfrA ) and XZL1720 (Δ lfrR ). Efflux takes place at 37°C in the presence of glucose and is inhibited by the efflux inhibitors thioridazine and verapamil. EtBr was used at ½ MIC for each strain in order to ensure maximum EtBr-loading of the bacteria, without compromising cellular viability. CPZ, chlorpromazine; EPI, efflux pump inhibitor; TZ, thioridazine; VP, verapamil.