Skip to main content

Table 2 Effect of gadXY on btuB promoter

From: Repression of btuB gene transcription in Escherichia coliby the GadX protein

Plasmid

β-galactosidase activitya

% inhibitionb

(A): pGAD10 + pC-lacZ

0

 

(B): pGadXY + pC-lacZ

0

 

(A): pGAD10 + pCB461lacZ

6.4 ± 0.2

45.7

(B): pGadXY + pCB461lacZ

3.5 ± 0.2

 

(A): pGAD10 + pCB673lacZ

7.2 ± 0.1

47.1

(B): pGadXY + pCB673lacZ

3.8 ± 0.1

 

(A): pGAD10 + pCB913lacZ

4.8 ± 0.2

54.5

(B): pGadXY + pCB913lacZ

2.2 ± 0.5

 

(A): pGAD10 + pCB1285lacZ

37.5 ± 0.7

56.7

(B): pGadXY + pCB1285lacZ

16.2 ± 0.5

 
  1. aMiller unit.
  2. bCaculated according to the following equation: 1- [β-galactosidase activity of (B) ÷ β-galactosidase activity of (A)] × 100%.
Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us