Figure 1
From: Repression of btuB gene transcription in Escherichia coliby the GadX protein
Structures of pGAD10, pGadXY, pGadX, and pGadY. pGAD10 was the vector used to clone gadXY, gadX, and gadY. pGadXY has a 1,470-bp fragment containing gadX, gadY, and a portion of gadW of E. coli K-12 genomic DNA inserted into the EcoRI site of pGAD10. pGadX contains a DNA fragment carrying the 825-bp gadX also inserted into the EcoRI site of pGAD10. pGadY is derived from pGadXY by deleting the 601-bp NcoI-DraIII fragment and thus contains a truncated gadX, the entire gadY, and a portion of gadW. Nucleotide sequences of the promoter regions of gadX and gadY are shown. The orientation of gadX is opposite to that of gadY. The sigma factor S (RpoS) recognition site and the Shine-Dalgarno (SD) sequence are shown in the 5' end region of gadX. PADH is the promoter of GAL4-AD and is not functional in E. coli.