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Table 2 The bacterial strains used in this study

From: Improving Salmonella vector with recmutation to stabilize the DNA cargoes

Strain

Genotype* [parental strain]

Reference or source

S. Typhimurium UK-1

  

   χ3761

wild type

[62]

   χ9833

ΔrecA62 [χ3761]

This study

   χ9070

ΔrecF126 [χ3761]

This study

   χ9072

ΔrecJ1315 [χ3761]

This study

   χ9081

ΔrecJ1315 ΔrecF126 [χ9072]

This study

   χ9931

cysG494::(5'tet-kan-3'tet) [χ3761]

This study

   χ9932

ΔrecF126 cysG494::(5'tet-kan-3'tet) [χ9070]

This study

   χ9933

ΔrecJ1315 cysG494::(5'tet-kan-3'tet) [χ9072]

This study

   χ9934

ΔrecA62 cysG494::(5'tet-kan-3'tet) [χ9833]

This study

   χ9935

cysG493::(5'tet-kan) [χ3761]

This study

   χ9936

ΔrecF126 cysG493::(5'tet-kan) [χ9070]

This study

   χ9937

ΔrecJ1315 cysG493::(5'tet-kan) [χ9072]

This study

   χ9938

ΔrecA62 cysG493::(5'tet-kan) [χ9833]

This study

   χ9939

ΔrecF126 Δ recA62 [χ9070]

This study

S. Typhi Ty2

  

   χ3769

wild type

[63]

   χ11053

ΔrecF126 [χ3769]

This study

   χ11134

ΔrecF1074 [χ3769]

This study

   χ11159

ΔrecA62 [χ3769]

This study

   χ11194

ΔrecJ1315 [χ3769]

This study

S. Typhi ISP1820

 

   χ3744

wild type

D.M. Hone

   χ11133

ΔrecF1074 [χ3744]

This study

S. Paratyphi A

  

   χ8387

Plasmid pSPA1 was cured from wt isolate ATCC 9281

This study

   χ11243

ΔrecA62 [χ8387]

This study

   χ11244

ΔrecF126 [χ8387]

This study

   χ11245

ΔrecJ1315 [χ8387]

This study

E. coli K-12

  

   EPI300

F- mcrA Δ (mrr-hsdRMS-mcrBC) Φ80dlacZ Δ M15 Δ lacX74 recA1 endA1 araD139 Δ (ara, leu)7697 galU galK λ- rpsL nupG trfA dhfr

Epicentre

   χ7213 (MGN-617)

thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc::Muλpir ΔasdA4 Δzhf-2::Tn10

[55]

  1. * kan: kanamycin resistance gene; 5'tet: 5' portion of the tetA gene together with its promoter; 3'tet: 3' portion of the tetA gene.