Table 1 Plasmids used in this study
From: Improving Salmonella vector with recmutation to stabilize the DNA cargoes
Plasmid | Relevant characteristic(s)* | Reference or source |
|---|---|---|
pACYC184 | cat, tetA, p15A ori | [59] |
pBAD-HisA | amp, pBR ori | Invitrogen |
pKD46 | λ Red recombinase expression plasmid | [60] |
p15A-PB2-kan | cat, kan, p15A ori | This study |
pYA4463 | pACYC184, adjacent 5'tet and 3'tet | This study |
pYA4464 | pACYC184, 3'tet | This study |
pYA4465 | pBAD-HisA; 5'tet | This study |
pYA4590 | pACYC184, 5'tet-kan-3'tet | This study |
pYA4373 | cat-sacB | [54] |
pRE112 | oriT, oriV, sacB, cat | [61] |
pYA3886 | pRE112, ΔrecF126 | This study |
pYA4783 | pYA3886, ΔrecF1074 | This study |
pYA3887 | pRE112, ΔrecJ1315 | This study |
pYA4680 | pRE112, ΔrecA62 | This study |
pYA4518 | pYA4464, cat, p15A ori, GFP gene | This study |
pYA4518-cysG | Two cysG fragments | This study |
pYA4689 | pYA4518-cysG, 5'tet-kan-3'tet | This study |
pYA4690 | pYA4518-cysG, 5'tet-kan | This study |
pYA5001 | aacC1, pSC101 ori, T vector | This study |
pYA5002 | pYA5001, recA cassette from Typhimurium χ3761 | This study |
pYA5004 | pYA5001, recA cassette from Typhi Ty2 χ3769 | This study |
pYA5005 | pYA5001, recF gene from Typhimurium χ3761 | This study |
pYA5006 | pYA5001, recF gene from Typhi Ty2 χ3769 | This study |