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Table 1 Primers used for detection of TEM and SHV β-lactamases and for site directed mutagenesis in this study

From: Enzymatic analysis of the effect of naturally occurring Leu138Pro mutation identified in SHV β-lactamase on hydrolysis of penicillin and ampicillin

Targets

Primer

Sequence (5'-3')

Product

size(bp)

Annealing

temp

Gene bank

Accession no.

TEM

TEM-F TEM-R

TCG GGG AAA TGT GCG TGC TTA ATC AGT GAG GCA CC

1074

62

AM849806

SHV

SHV-F SHV-R

GCC GGG TTA TTC TTA TTT GTC GC ATG CCG CCG CCA GTC A

1016

62

EU342351

SHV-Ma

SHV-MF SHV-MR

C AAT CTG CCG CTG GCC ACC GTC GGC GGC CAG CGG CAG ATT GGC GGC GCT G

 

52

-

SHV-33b

SHV-33F SHV-33R

GTG CTG TCT GCG GGC TGG TTT ATC GCC CGC AGA CAG CAC GGA GCG GAT C

 

52

-

  1. a : primer used to create the mutation L138P in SHV-1 β-lactamase identified in this study
  2. b: primer used to create a single mutation P226S (SHV-33 β-lactamase)