Figure 3From: The promoter of filamentation (POF1) protein from Saccharomyces cerevisiae is an ATPase involved in the protein quality control processPof1p purification and activity analyses. (A) SDS-PAGE showing the purification of recombinant Pof1p obtained through metal affinity chromatography. Lane 1: molecular weight standard; subsequent lanes were different fractions obtained during the elution process. (B) Thin layer chromatography analyses to observe Pof1p ATP transferase activity; the controls were included to assay for alterations in CTP and ATP. See the Materials and Methods section for details.Back to article page