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Table 4 Transformation with the HP1 knockout construct

From: Transformation of Botrytis cinerea by direct hyphal blasting or by wound-mediated transformation of sclerotia

 

Blast

Sclerotia

Experimental material

Mycelium1

Sclerotia

Quantity per experiment2

4

20

Transformants3

30%

15%

  1. 1On PDA plates.
  2. 2Number of plates used for blasting. Ten plugs were excised from each plate resulting in 40 isolates subjected to Hyg selection.
  3. 3Verified by Hyg selection and PCR.
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BMC Microbiology

ISSN: 1471-2180

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