| Blast | Sclerotia | Electroporation |
---|
Experimental material | Mycelium1 | Sclerotia | Cells2 |
Quantity per experiment3 | 10 | 45 | 3 x106 |
Transformants4 | 39% | 46% | 0 |
Putative knockouts5 | 54% | 62% | 0 |
- 1On PDA plates.
- 2Protoplasts generated from broken hyphae, germinating conidia or both.
- 3Number of plates used for blasting. Ten plugs were excised from each plate resulting in 100 isolates subjected to Hyg selection.
- 4Verified by Hyg selection and PCR.
- 5Homologous recombination verified by PCR and sequencing.