Table 1 Primer details for the PCR analysis of transformants
No. | Name | Sequence | Fragment size (bp) |
|---|---|---|---|
1 | Hyg F | CGACGTTACTGGTTCCCGGT | Â |
2 | Hyg R | GCGGGCACGTTAACTGAT | 550 |
3 | bR-gen 5'F | ACAAGACCTCTCGCCTTT | Â |
4 | bR-Hyg 5'R | AGGTCGGAGACGCTGTCGAA | 480 |
5 | bR-gen 3'F | ATGCAGCTTGGGCTGTTCAG | Â |
6 | bR-Hyg 3'R | CGACTCCCAACTCGACTA | 590 |
7 | Phleo F | GGGGACAAGTTTGTACAAAAAAGCAGGCT | Â |
8 | Phleo R | GGGGACCACTTTGTACAAGAAAGCTGGGT | 1020 |