Figure 2

PCR analyses of transformants of B. cinerea . and S. sclerotiorum. (a) A fragment of the Hyg^r cassette (550 bp) was amplified by primers 1 and 2 from five different bR knockout strains (1-5). A 480-bp fragment was amplified by primer 3 which is located in the 5' upstream genomic region of the bR gene and by primer 4 in the Hyg cassette (5'), and a 590-bp fragment was amplified by primer 5 which is located in the 3' downstream genomic region of the bR gene and primer 6 which is located at the 3' end of the Hyg cassette (3'); P is the positive control of the bR knockout construct (plasmid DNA). (b) A fragment of the Phleo^r cassette (1020 bp) was amplified by primers 3 and 4 from four different bR complementation strains (1-4). C is the negative control of the WT strain. (c) A fragment of the Hyg^r cassette (550 bp) was amplified by primers 1 and 2 from the HP1 transformants (1-7). C is the negative control of the WT strain. (d) A fragment of the Hyg^r cassette (550 bp) was amplified by primers 1 and 2 from four transformants of S. sclerotiorum (1-4). P is the positive control of the Hyg^r cassette (plasmid DNA) and C is the negative control of the WT strain (primers sequences are listed in Table 1).