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Table 3 Protective effect of Δfn r mutation for cell killing initiated by mutant topoisomerase I cleavage complex accumulation under aerobic and low oxygen conditions

From: Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR

Survival Ratio
  Aerobic Low Oxygen
BW27784 1.18 × 10-4 ± 7.7 × 10-5 1.07 × 10-3 ± 4.7 × 10-4
IFL6 1.30 × 10-3 ± 3.1 × 10-4 8.15 × 10-2 ± 3.1 × 10-3
Δfnr effect 11-fold 76-fold
  1. Cultures were grown to exponential phase without shaking, then treated with 0.002% arabinose for 2.5 h under either aerobic or low oxygen conditions before serial dilution and plating on LB plates with antibiotics and 2% glucose. Survival ratio was determined by calculating the ratio of the viable colony counts obtained from the induced cultures versus the viable counts from non-induced culture. The results represent the average and standard errors from at least three experiments