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Table 1 Effect of high copy plasmid clones on survival following accumulation of mutant topoisomerase I cleavage complex

From: Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR

Plasmid

Survival Ratio

pCRII vector

7.85 × 10-5 ± 1.19 × 10-5

pAQ5

4.95 × 10-3 ± 1.55 × 10-3

pAQ5-1

4.92 × 10-3 ± 1.20 × 10-3

pAQ5-2

1.25 × 10-2 ± 2.48 × 10-3

pInter

1.90 × 10-2 ± 4.12 × 10-3

pInterD1

4.22 × 10-3 ± 1.02 × 10-3

pInterD2

5.19 × 10-4 ± 1.73 × 10-4

  1. E. coli BW27784 carrying pAYTOP128 was transformed with high copy number plasmid shown in the table. Cultures were grown to exponential phase with shaking, then treated with 0.002% arabinose for 2.5 h before serial dilution and plating on LB plates with antibiotics and 2% glucose
  2. Survival ratio was determined by calculating the ratio of the viable colony counts obtained from the induced cultures versus the viable counts from non-induced culture. The results represent the average and standard errors from at least three experiments
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BMC Microbiology

ISSN: 1471-2180

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