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Figure 6 | BMC Microbiology

Figure 6

From: Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR

Figure 6

Effect of Δ purR and Δ fnr mutations on sensitivity to topoisomerase I cleavage complex Strains BW27784 and its isogenic derivatives IFL6 (Δ fnr ), IFL7 ( ΔpurR ) were transformed with pAYTOP128 and grown in LB with shaking to exponential phase before the addition of 0.002% arabinose. (a) Viable colony counts of arabinose treated cultures were divided by the colony counts from the untreated culture to obtain the survival ratio. (b) Western blot analysis showed that the ΔpurR and Δfnr mutations did not affect expression levels of mutant YpTOP after induction with 0.002% arabinose for 2.5 h.

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