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Table 1 Integrity of antibiotic-treated bacteria

From: Contribution of bacterial outer membrane vesicles to innate bacterial defense

   WC (ng/mL) OMV-free Supe (ng/mL) AP Leakage ([AP] supe :[AP] whole cell ) a
Strain Treatmentb UNT TRE UNT TRE Untreated Treated
MK318 Polymyxin B (0.75 μg/mL) 8.270 ± 1.010 7.870 ± 0.970 1.290 ± 0.080 1.341 ± 0.121 0.160 ± 0.007 0.170 ± 0.006
  Colistin (0.5 μg/mL) 9.440 ± 0.230 8.87 ± 0.07 1.20 ± 0.010 1.260 ± 0.021 0.127 ± 0.003 0.121 ± 0.002
ETEC Polymyxin B (3 μg/mL) 6.100 ± 0.440 6.07 ± 0.510 1.201 ± 0.030 1.22 ± 0.030 0.198 ± 0.009 0.204 ± 0.020
ADA600 Untreated 0.020 ± 0.011 ND 0.024 ± 0.013 ND ND ND
  1. aRFU measurements of AP in the OMV-free culture supernatant (Supe) compared to AP in whole cell (WC) pellets, normalized to CFU/mL in the culture. No significant differences in AP leakage between untreated (UNT) and treated (TRE) cultures were observed (p > 0.05).
  2. bTreatments were for 2 h at 37°C; final concentration of treatments are shown. (n = 9)